Animal Cell Culture Bioreactors

Animal cell culture bioreactors initially employed stirred tanks containing.

Animal cell culture bioreactors.

Bioreactors play a key role in the field of biologics where they are used for the production of recombinant therapeutic proteins by large scale cultivation of animal cells. Cell cultures are particularly susceptible to biological contamination. This process can either be aerobic or anaerobic these bioreactors are commonly cylindrical ranging in size from litres. Microcarriers with adherent cells which were in principle an adaptation of homogeneous culture systems used for.

However despite this sparged bioreactors remain the preferred means of cell culture because sparging is a robust method of supplying oxygen especially on a large scale. The new bioreactor called the see saw bioreactor is ideal for the growth of cells with a sensitive membrane. There are several types of bioreactors including stirred tank airlift hollow fiber and rotary cell culture system rccs d. Purpose of this article is to review the current status of bioreactor design for mammalian cell culture.

In one case a bioreactor is a vessel in which a chemical process is carried out which involves organisms or biochemically active substances derived from such organisms. One bacterial spore or vegetative bacterial cell is all it takes to overgrow an animal cell culture. The new bioreactor called the see saw bioreactor is ideal for the growth of cells with a sensitive membrane. The gas sparging of culture broth causes damage to suspended animal cells.

Morphological and biochemical features of two major mammalian cell groups. Animal cell culture control for bioprocess engineering presents the design fabrication and control of a new type of bioreactor meant especially for animal cell line culture. This article examines the underlying mechanisms involved in bubble associated cell damage and the methods available for controlling such damage. It addresses engineering aspects such as mass transfer instrumentation and control ensuring successful design and operation of animal cell bioreactors.

Critical contamination points for a bioreactor are the transitions between sterile spaces such as the stirrer ports and gas lines. Requirements for a bioreactor for animal cell culture 1 well controlled environment t ph do nutrients and wastes 2 supply of nutrients 3 gentle mixing avoid shear damage to cells 4 gentle aeration add oxygen slowly to the culture medium but avoid the formation of large bubbles which can damage cells on contact.